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Screening for scab resistance by RAPD markers in cultivars of apple ( Malus spp.)
Author(s) -
Marn M. Viršček,
ŠTampar F.,
Javornik B.
Publication year - 1996
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1996.tb00963.x
Subject(s) - cultivar , biology , rapd , malus , genetic marker , gene , horticulture , botany , genetics , genetic diversity , population , demography , sociology
Genetic markers are a much faster and more practical alternative to classical methods for the identification of genes for scab resistance present in different apple cultivars. In our study, 28 scab‐resistant cultivars, four wild sources of the resistance genes and 10 susceptible cultivars were screened for the presence of the RAPD fragments OPM18/900, OPD20/600 and OPA15/900, which are reported to be linked to the Vf gene. All three marker fragments were successfully amplified with different protocols in Vf ‐resistant cultivars including ‘ M. floribunda 821’. No marker fragments were amplified in susceptible cultivars, three out of four Va ‐resistant cultivars, three out of four Vm ‐resistant cultivars, two Vr ‐resistant cultivars, ‘Antonovka PI 172612’ and ‘ M. pumila R 12740‐7A’. All three markers were found in the cv. ‘Nova Easygro’, reported to possess the Vr gene, and the cv. ‘Reglindis’, reported to be Va ‐resistant. M. atrosanguinea of unknown origin showed the presence of OPD20/600 and OPA 15/900 marker bands. The cvs. ‘Nova Easygro’, ‘Reglindis’ and M. atrosanguinea are probably carriers of the VF gene.