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RAPD‐mapping of the distal portion of chromosome 3 of barley, including the BaMMV/BaYMV resistance gene ym4
Author(s) -
Weyen J.,
Bauer E.,
Graner A.,
Friedt W.,
Ordon F.
Publication year - 1996
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1996.tb00920.x
Subject(s) - rapd , bulked segregant analysis , biology , genetics , doubled haploidy , locus (genetics) , ploidy , gene , genetic linkage , gene mapping , chromosome , population , demography , sociology , genetic diversity
Experiments were carried out in order to identify RAPD‐markers for the BaMMV/BaYMV resistance gene ym4 to facilitate efficient marker‐based selection in practical breeding programmes. Linkage analysis was carried out with 283 doubled haploid (DH) barley lines out of a cross between the BaMMV/BaYMV susceptible cv. ‘Igri’ and the resistant cv. ‘Franka’. Using bulked segregant analysis, 310 RAPD‐primers were screened for polymorphism between the parents. Four of these primers gave rise to specific bands linked to the resistance gene ym4.