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Use of electrophoresis for variety testing in carrot
Author(s) -
Lallemand J.,
Briand F.
Publication year - 1995
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1995.tb01255.x
Subject(s) - biology , electrophoresis , selection (genetic algorithm) , enzyme , botany , biochemistry , computer science , artificial intelligence
Electrophoretic markers were used to describe varieties of carrot and tested for their use in distinguishing and identifying them. Seed proteins and ten enzyme systems (PGI, PGM, PGD, ACP, ADH, GOT, IDH, MDH, MR and SkDH) were studied in a set of varieties and selection lines. Using the proteins from bulk samples of more than 25 seeds per variety, consistent results were obtained which enabled the 38 varieties studied to be divided into 14 groups. Among the enzymes studied, PGI, PGM and PGD were found to be convenient for distinction purposes. They demonstrated the heterogeneity of the varieties and their combined use enabled most of them to be distinguished from one another. The use of electrophoretic markers for distinguishing varieties is discussed.