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Physical mapping of differences between the chloroplast DNAs of Beta vulgaris and B. webbiana
Author(s) -
Kishima Y.,
Yanai Y.,
Kinoshita T.,
Mikami T.
Publication year - 1995
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1995.tb01251.x
Subject(s) - biology , genome , open reading frame , genetics , restriction map , chloroplast dna , inverted repeat , chloroplast , restriction site , restriction fragment , cleavage (geology) , microbiology and biotechnology , dna , restriction enzyme , gene , nucleic acid sequence , peptide sequence , paleontology , fracture (geology)
A physical map of Beta webbiana (a wild species of the section Pro‐cumbentes ) chloroplast genome was constructed by localizing the cleavage sites of Sma I, Pst I, Pvu II, Xho I, and Hind III, and the map was then aligned with the map of sugarbeet ( B. vulgaris ) chloroplast DNA. This alignment shows 27 restriction‐site changes and 11 insertions/deletions, most of which occur in the large single‐copy region of the genome. A 0.7‐kb long mutation, located within an unidentified open reading frame (ORF2280) in the inverted repeat, was also found.