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In vitro selection for modified amino‐acid pathways in Triticum species
Author(s) -
Lange F. D.,
Brown P. T. H.,
Kollmorgen J. F.,
Lörz H.
Publication year - 1995
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1995.tb01248.x
Subject(s) - acetolactate synthase , biology , callus , amino acid , mutant , biochemistry , tryptophan , in vitro , cysteine , enzyme , threonine , biosynthesis , valine , lysine , botany , serine , gene
In vitro selections were carried out to obtain mutant plants of Triticum aestivum L. and T. monococcum L. with modified amino‐acid pathways. Embryogenic callus cultures were selected with the amino acids L‐lysine plus L‐threonine and the amino acid analogues S‐2‐aminoethyl‐L‐cysteine, 5‐methyl‐L‐tryptophan and sulfometuron‐methyl. A number of plants were regenerated from these selections and tested for resistance at the whole‐plant level. Only plants from the selections with sulfometuron‐methyl proved to be resistant and these were characterized biochemically. Three different mutant lines could be detected. The resistance of these plants could be associated with an altered enzyme. Acetolactate synthase was less sensitive to inhibition. A discussion of the suitability of the selection agents is presented.