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Direct plant regeneration from leaf explants in cucumber ( Cucumis sativus L.) is free of stable genetic variation
Author(s) -
Burza W.,
Malepszy S.
Publication year - 1995
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1995.tb01246.x
Subject(s) - cucumis , explant culture , biology , botany , regeneration (biology) , cucurbitaceae , greenhouse , horticulture , plant growth , in vitro , genetics
A new procedure is described for rapid and efficient plant regeneration from leaf explants of Cucumis sativus and C. anguria. The following factors were most important: young leaves, the NO 3 : NH 4 ratio in the plant induction medium, small explants and the growth regulator combination. After about 4 weeks of culture under optimal growth conditions, the frequency of regeneration was 10–100% of explants. Six to 7 weeks were required to obtain well‐rooted plants, which were mostly able to survive after transfer into soil. From a single young leaf of C. sativus cv. ‘Borszezagowski’, 135 plants could be regenerated. All plants transferred to a greenhouse were free of morphological or physiological abnormalities, flowered normally and bore fruits. The analysis of R 0 plants showed no genetic variation, whereas in the R 1 two new phenotypes, which were not transmittable to the R 2 , were observed. This procedure is recommended for its production of homogeneous cucumber plants.