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The HMW glutenin suhunit composition of bread‐wheat varieties bred in Pakistan
Author(s) -
Tahiru M.,
Hussain S. A.,
Turchetta T.,
Lafiandram D.
Publication year - 1995
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1995.tb00828.x
Subject(s) - glutenin , biology , protein subunit , genome , composition (language) , genotype , genetics , polyacrylamide gel electrophoresis , food science , gene , microbiology and biotechnology , biochemistry , enzyme , linguistics , philosophy
A total of 50 bread‐wheat varieties bred in Pakistan were characterized for the composition of the high‐molecular‐weight glutenin subunit (HMW‐GS). The glutenin subunits, as revealed by sodium dodecyl‐sulfate‐polyacrylamide‐gel electrophoresis (SDS‐PAGE) were numbered according to Payne's numbering system and, varieties were accordingly assigned theoretical quality scores. All varieties were found to possess either subunit 1 or 2* coded by the A genome. Subunits 17+18 and 7 + 9 of the B genome and 5+10 of the D genome were predominantly found in this set of varieties. The frequency of the appearance of Glu‐l alleles in the varieties was different from that seen in other countries, especially in terms of the absence of the ‘null’ form of the A genome and the presence of novel subunits at the Glu‐Bl and Glu‐Dl loci. The compositions of HMW‐GS were generally of good quality, with more than 50% of the varieties achieving quality scores of 9–10 with a high (8.6) average score. The results in this study indicate that wheat varieties bred in Pakistan have a narrow genetic base in terms of HMW glutenin subunits. It appears that the breeders have been selecting wheat genotypes of glutenin subunits associated with good quality without knowing the actual composition of these subunits. The database established on the basis of these results is useful for wheat‐improvement programmes aimed at varietal identification and breeding for good quality parameters.