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Restriction analysis of the mitochondrial DNA of male‐sterile and maintainer lines of pearl millet, Pennisetum americanum L.
Author(s) -
Munjal S. V.,
Narayan R. K. J.
Publication year - 1995
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1995.tb00806.x
Subject(s) - software maintainer , biology , southern blot , restriction enzyme , mitochondrial dna , cytoplasmic male sterility , pennisetum , genetics , gene , restriction fragment length polymorphism , microbiology and biotechnology , genome , polymerase chain reaction , botany
Mitochondrial DNA from two pairs of cytoplasmic male‐sterile (cms) and maintainer lines of pearl millet was investigated by restriction‐enzyme analysis and Southern‐blot hybridization using three mitochondrial gene probes. Each pair of male‐sterile and maintainer lines was of a different nuclear origin. The objective was to distinguish differences in the DNA base‐sequence organization of the mitochondrial genomes of cms and maintainer lines from the two sources. Restriction‐enzyme analysis revealed differences between the different cms and maintainer lines. Southern‐blot hybridization experiments using cloned mitochondrial gene probes further distinguished differences between different lines. It is expected that the restriction‐fragment‐length polymorphisms revealed in the Southern‐blot‐hybridization experiments will be useful in distinguishing and classifying cms and maintainer lines obtained from different nuclear backgrounds.