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Chromosome doubling in Tripsacum : the production of artificial, sexual tetraploid plants
Author(s) -
Leblanc O.,
Dueñas M.,
Hernández M.,
Bello S.,
Garcia V.,
Berthaud J.,
Savidan Y.
Publication year - 1995
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1995.tb00798.x
Subject(s) - apomixis , biology , ploidy , polyploid , botany , chromosome , chromosome number , colchicine , genetics , karyotype , gene
A collection of embryogenic diploid calli of Tripsacum was established and treated with colchicine to induce chromosome doubling. Sections containing duplicated cells in calli were identified using flow cytometry and ploidy level was determined in the regenerated plantlets. Tetraploid plants from several origins were obtained. In contrast to wild polyploid plants, which show apomictic development, the regenerated tetraploid plants reproduced sexually. By hybridizing these plants with wild tetraploid apomicts, various populations were established; these will allow a study of the inheritance of apomixis in Tripsacum.