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Purity control of F 1 ‐hybrid tomato cultivars by RAPD markers
Author(s) -
Rom M.,
Bar M.,
Rom A.,
Pilowsky M.,
Gidoni D.
Publication year - 1995
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1995.tb00790.x
Subject(s) - rapd , lycopersicon , biology , cultivar , hybrid , genetic marker , horticulture , microbiology and biotechnology , genetics , botany , gene , genetic diversity , population , demography , sociology
Randomly amplified polymorphic DNA (RAPD) markers were applied in purity control of hybrid seed production of tomato ( Lycopersicon esculentum Mill.). DNA from three commercial F 1 ‐hybrid cultivars and their parental lines was subjected to RAPD screening with 50 primers. Two of four primers which detected polymorphism between the parents tested, generated paternal‐specific RAPDs, enabling a clear distinction to be made between hybrids and their maternal parents. In addition, combination of the polymorphic DNA products generated by these primers exhibited hybrid‐specific patterns, enabling each cultivar to be identified. This result indicates the practical usefulness of RAPD markers in hybrid‐tomato‐seed purity‐control tests and cultivar identification. The approach is advantageous in its rapidity and simplicity, particularly as an alternative for those cultivars for which lengthy and costly phenotypic tests are currently used.