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Regeneration of Fertile Plants from Cell‐suspension‐derived Small‐cell Colonies of Lolium perenne (L.)
Author(s) -
Altepeter F.,
Posselt U. K.
Publication year - 1994
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1994.tb00744.x
Subject(s) - biology , lolium perenne , regeneration (biology) , suspension culture , botany , suspension (topology) , lolium , cell , cell wall , filtration (mathematics) , cell culture , microbiology and biotechnology , poaceae , biochemistry , genetics , mathematics , homotopy , pure mathematics , statistics
Abstract A defined method for the rapid establishment of cell suspensions releasing small‐cell colonies and their regeneration to fertile plants is described. Cell suspensions were obtained from 10 mature, lengthwise‐divided embryos. Five of the 10 suspensions regenerated green plants. Filtration of suspensions through a 150 μm mesh followed by regeneration was carried out 28 days after initiation of the suspensions, to demonstrate a rapid release of regenerable small cell colonies from cell suspensions using the protocol described. Two of the 10 filtered suspensions regenerated green plants from small cell colonies.