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Genetic ‘Fingerprinting’ of Sunflower Lines and F 1 Hybrids using. Isozymes, Simple and Repetitive Sequences as Hybridization Probes, and Random Primers for PCR
Author(s) -
Mosges G.,
Friedtu W.
Publication year - 1994
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1994.tb00713.x
Subject(s) - biology , isozyme , sunflower , hybrid , genotype , genetics , microsatellite , genetic variability , dna profiling , microbiology and biotechnology , genetic marker , gene , allele , dna , botany , enzyme , horticulture , biochemistry
Three different marker systems which have been widely described in the characterization of genotypes were compared. Sunflower lines and hybrids were analysed by three isozymes, oligonucleotides (GACA) 4 , (GATA) 4 , and (GGAT) 4 as hybridization probes, and 70 primers for PCR analysis. Polymorphisms were visible with each type of marker. However, the repeated probes, especially (GATA) 4 , revealed the highest degree of variability and seem to be most appropriate for the generation of genotype‐specific ‘fingerprints.’