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Isoenzyme Electrophoresis, a Simple Way to Identify 1B/1R Substitutions and Translocations in Wheat
Author(s) -
Hartmann H.,
Schiele S.,
Lelley T.
Publication year - 1994
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1994.tb00693.x
Subject(s) - biology , isozyme , malate dehydrogenase , chromosomal translocation , electrophoresis , starch , genetics , biochemistry , chromosome , isomerase , enzyme , gel electrophoresis , microbiology and biotechnology , gene
In order to identify 1B/1R translocations and substitutions by simple means in breeding materials, the efficiency of using starch gel electrophoresis of isoenzymes was evaluated. GPI (glucose phosphate isomerase) and MDH (malate dehydrogenase) were tested in 17 lines of wheat which had previously been identified for their 1B/1R status by C‐banding. By electrophoresis of these two enzymes the rye substitution of only one wheat chromosome arm could be identified with high accuracy.