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Plant Regeneration Through Anther Culture of Three Wild Species of Hordeum ( H. murinum, H. marinum and H. bulbosum )
Author(s) -
Kihara M.,
Fukuda K.,
Funatsuki H.,
Kishinami I.,
Aida Y.
Publication year - 1994
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1994.tb00678.x
Subject(s) - biology , microspore , botany , stamen , shoot , hordeum , murashige and skoog medium , tissue culture , hordeum vulgare , poaceae , pollen , biochemistry , in vitro
Plant regeneration was achieved through anther culture of three wild species of Hordeum (H. murinum, H. marinum and H, bulbosum ). Calli or embryoids were formed from microspores in anthers cultured on a medium containing 6‐benzylammopurine (BAP) and ficoll. These calli or embryoids regenerated green or albino shoots and roots after transfer to regeneration media. Green plantlets which developed on regeneration media were transferred to soil where they showed further growth.