Premium
Development of a New Cytoplasmic Male‐Sterility System in Brassica juncea through Wide Hybridization
Author(s) -
Rao G. U.,
BatraSarup Vinita,
Prakash S.,
Shivanna K. R.
Publication year - 1994
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1994.tb00666.x
Subject(s) - biology , brassica , microspore , cytoplasmic male sterility , sterility , stamen , petal , botany , cultivar , meiosis , ploidy , pollen , genetics , gene
A new cytoplasmic male‐sterility system was developed in an oilseed Brassica , viz. B. juncea var. ‘Pusa Bold’ with the cytoplasmic background of a wild species, Diplotaxis siifolia , obtained through wide hybridization. The synthetic alloploid ( D. siifolia × B. juncea : 2n = 56, D 3 D 3 AABB) was repeatedly backcrossed to B. juncea to achieve cytoplasmic substitution. The CMS plants resembled the cultivar in growth and morphology. The flowers had narrow sepals and petals and short, shrivelled anthers which failed to dehisce. The meiotic process appeared to be normal. The microspores degenerated at an early stage after tetrad formation. Female fertility in the CMS plants was as good as in the cultivar. Female transmission of sterility confirmed it to be cytoplasmically encoded.