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Induction of Tetraploidy in the Tanier Cultivar ‘Inglesa’ [ Xanthosoma saghtifolium (L.) Schott]
Author(s) -
Esnard J.,
Ferwarda F.,
RiveraAmador E.,
Hepperly P. R.
Publication year - 1993
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1993.tb00651.x
Subject(s) - biology , sucrose , colchicine , tissue culture , cultivar , botany , ploidy , horticulture , araceae , in vitro , food science , biochemistry , gene , genetics
Induction of tetraploidy in commercial tanier was successfully achieved in vitro. Tissue cultures of a tanier [ Xanthosoma sagittifolium (L.) Schott cv. ‘In‐glesa’, 2n 26] were established and maintained on modified nutrient media containing various concentrations of MURASHIGE and SKOOG basal salts, vitamins, sucrose, BA and IAA or 2,4‐D. Cultures were exposed continuously for 8 days to 0, 12.5, 25 and 50 mg/1 colchicine incorporated in the media. The colchicine‐treated tissue cultures yielded di‐ploids (2n 26), tetraploids (2n 52) and aneuploids (2n – 2 24) respectively. The diploids produced on average one leaf and 4 suckers more per plant than the tetraploids, while plant height increased at about the same rate. Continuous exposure of tanier tissue cultures tocolchicine is an effective method for the production of synthetic tetraploids that may be hybridized with non‐commercial root rot‐resistant tetraploids.