Premium
Chromosomal Assignment of Three Enzyme Coding Loci ( Icd1, Nad‐Mdh1 and Aco1 ) Using Primary Trisomics in Beet ( Beta vulgaris L.)
Author(s) -
Lange W.,
Oleo M.,
Bock Th. S. M.,
D'Haeseleer M.,
Jacobs M.
Publication year - 1993
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1993.tb00627.x
Subject(s) - biology , locus (genetics) , genetics , nondisjunction , isocitrate dehydrogenase , ploidy , drosophila pseudoobscura , isozyme , microbiology and biotechnology , backcrossing , chromosome , enzyme , gene , aneuploidy , biochemistry
Chromosomal assignment of three enzyme coding loci was established by evaluation of the segregation distortion in the offspring of crosses between heterozygous primary trisomics of a complete series in beet ( Beta vulgaris ) and heterozygous diploid pollinators. Depending on the rate of isozyme polymorphism in the original plant material, at least one, but more often two, crossing cycles were needed to obtain the desired segregating populations. In one case, a backcross was used to confirm the segregation distortion, and in two cases, the chromosomal assignment of the isozyme loci was confirmed by studying the dosage shift in the electrophoretic pattern of the critical trisomics. The isocitrate dehydrogenase locus ( Icd1 ) is situated on chromosome II, the NAD‐dependent malate dehydrogenase locus ( Nad‐Mdh1 ) on chromosome III, and the aconitase locus ( Aco1 ) on chromosome IV.