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Segregation Analysis of Isozyme Markers on Isolated Microspore‐Derived Embryos in Brassica napus L.
Author(s) -
Foisset N.,
Delourme R.,
Lugas M. O.,
Renard M.
Publication year - 1993
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1993.tb00595.x
Subject(s) - isozyme , biology , microspore , mendelian inheritance , locus (genetics) , rapeseed , hybrid , genetics , gene , brassica , doubled haploidy , ploidy , genotype , genetic marker , embryo , botany , enzyme , stamen , biochemistry , pollen
The segregation of isozyme genetic markers was studied on embryos arising from isolated microspore cultures in live rapeseed F1 hybrid genotypes. Out of the ten isozyme genes considered, live ( Aco‐1, Aco‐3, Lp‐1, Pgm‐3, Tpi‐1 ) did not segregate according to expected Mendelian ratios in at least two F1 crosses. F2 plants, generated from the same hybrids, included in the analysis, were tree of segregation distortions. Linkage analysis between each segregating enzyme locus in each progeny revealed independance between these markers. In this paper, the results of the linkage analysis as well as the origins of the distortions are discussed. The existence of androgenic embryogenesis genes may be responsible for the abnormal segregations of the five isozyme genes.