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A Rapid Screening Method for Testing the Resistance of Cotyledons to Downy Mildew in Brassica napus and B. campestris
Author(s) -
Gröuntoft M.
Publication year - 1993
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1993.tb00579.x
Subject(s) - downy mildew , biology , brassica , glucosinolate , leptosphaeria maculans , inoculation , cotyledon , agronomy , horticulture , botany
Several hundred breeding lines of summer and winter forms of turnip rape as well as winter rape, were sown on greenhouse tables filled with soil. A week later the cotyledons were artificially inoculated and covered with a plastic tunnel. Approximately two weeks later the average percentage of the wilted leaf area was estimated. The method is rapid and demands little labour. It is therefore suitable for testing large numbers of breeding lines. The response to Peronospora parasitica (Pers. ex Pers.) Fr. in the rape material varied from being highly susceptible to highly resistant. Only minor differences were detected between lines with high and low glucosinolate contents. All the turnip rape material had very high resistance to P. parasitica at the cotyledonous stage.