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The Effect of Gelling Agents on Wheat Anther and Immature Embryo Culture
Author(s) -
Simonson Randal L.,
Baenziger P. S.
Publication year - 1992
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1992.tb00175.x
Subject(s) - biology , ficoll , starch , agar , embryo , stamen , botany , sucrose , poaceae , endosperm , horticulture , food science , pollen , biochemistry , in vitro , microbiology and biotechnology , peripheral blood mononuclear cell , genetics , bacteria
This study was conducted to determine whether inexpensive starches and various agars could replace Ficoll, which is very expensive, and Bacto‐agar, which may be inhibitory, in wheat ( Triticum aestivum L.) anther and immature embryo culture. Anthers from Pavon 76’ had significantly greater embryoid‐initiation frequencies (number of embryoids per anther) on PI media with corn (0.67) and wheat starch (1.50) than with Ficoll (0.47). Anthers on 85D12 media with Ficoll, corn starch and wheat starch had similar embryoid‐initiation frequencies (0.51, 0.50, and 0.52, respectively). The embryoid‐regeneration frequencies (number of regenerating embryoids per embryoid plated) on 85D12 media containing corn (0.32) and wheat (0.36) starch were significantly higher than on 85D12 with Bacto‐agar (0.14). Embryoids on MS media with Bacto‐agar, corn starch, and wheat starch had similar embryoid‐regenerating frequencies (0.15, 0.20, 0.22, respectively), which were greater than with some of the other agars tested (0.05 to 0.17). When wheat starch replaced Bacto‐agar in immature embryo cultures, the frequency of responding embryos was unchanged, but the frequency of green plants regenerated increased an average of fourfold for two cultivars.