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Effect of Karyotype on Somatic Embryogenesis from Immature Triticale (× Triticosecale Wittmack) Embryos
Author(s) -
Immonen A. S. T.
Publication year - 1992
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1992.tb00161.x
Subject(s) - karyotype , biology , callus , somatic embryogenesis , embryo , triticale , somatic cell , chromosome , botany , embryogenesis , genetics , gene
To assess the tissue‐culture response of different karyotypes of spring triticales, immature embryo explants of 20 secondary triticales with a complete karyotype (complete rye genome with or without a 6D/6A substitution) or substituted karyotype (incomplete rye genome with a 2D/2R substitution), two primary triticales with octoploid and two with hexaploid karyotypes, were cultured on MS and on Kao medium supplemented with 2,4‐D. Complete triticales produced significantly more embryogenic callus than substituted types. Complete 6D/6A karyotypes showed the highest embryogenic response. Octoploid primary triticales showed high and uniform production of embryogenic callus on both media. These findings complement earlier results on the agronomic and quality differences between the karyotypes.