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Improved Culture System for Microspores of Barley to Become a Target for DNA Uptake
Author(s) -
Kuhlmann Ursula,
ForoughiWehr Bärbel,
Graner A.,
Wenzel G.
Publication year - 1991
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1991.tb00546.x
Subject(s) - microspore , biology , exogenous dna , transformation (genetics) , dna , hordeum vulgare , polyethylene glycol , cultivar , botany , gene , spore , poaceae , doubled haploidy , plasmid , protoplast , ploidy , stamen , biochemistry , pollen
For the winter barley cultivar ‘Igri’, a microspore isolation and regeneration procedure is described which allows the production of such vigorous micro‐spore fractions that this single‐cell system can be used as a target for DNA uptake. Up to 60 % of the vigorous microspores isolated from the anther, and cultured in liquid modified MS medium, formed embryoids and/or calli. Such preparations were used for trials in DNA uptake with the plasmid pBI 221. Transformation trials were performed with polyethylene glycol as the inducing agent. With this treatment, a relative increase of fluorescence could be shown under UV light indicative of transient expression of the uidA gene.