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Development of RFLP Markers for Barley
Author(s) -
Jahoor A.,
Backes G.,
Graner A.,
Herrmann R. G.,
Fischbeck G.
Publication year - 1991
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1991.tb00530.x
Subject(s) - biology , restriction fragment length polymorphism , hindiii , ecori , genetics , bamhi , hordeum vulgare , genomic dna , repeated sequence , dna , botany , poaceae , restriction enzyme , polymerase chain reaction , genome , gene
A PstI‐based genomic library from barley DNA was screened for RFLPs in the three relatively‐distant cultivars ‘Alexis’ (2‐row spring type), ‘Igri’ (2‐row winter type) and ‘Mammut’ (6‐row winter type), digested with BamHI, EcoRI and HindIII. 50 % of the 108 DNA fragments studied represented single‐copy sequences, 29 % low‐copy and 21 % repetitive sequences. The DNA probes were assigned to discrete barley chromosomes with the aid of wheat/barley addition lines. 80 % of the single‐ and low‐copy sequences hybridized with both barley and wheat DNA, whereas most repetitive sequences gave signals only with barley DNA.