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Transformation of Medicago sativa L. Using a Ti Plasmid Derived Vector
Author(s) -
Pezzotti M.,
Pupilli F.,
Damiani F.,
Arcioni S.
Publication year - 1991
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1991.tb00477.x
Subject(s) - biology , kanamycin , agrobacterium tumefaciens , transformation (genetics) , plasmid , medicago sativa , somatic embryogenesis , medicago , rhizobiaceae , agrobacterium , protoplast , ti plasmid , replicon , gene , botany , genetics , bacteria , symbiosis , embryogenesis
A cultivar of Medicago sativa was transformed with Agrobacterium tumefaciens strain At81 binary‐vector carrying the plasmid pKan3A, with the neomycin phosphotransferase gene under the control of the mannopine biosynthesis promoter. Stem segments were infected with the bacterium and kanamycin resistant calli were obtained; plant regeneration via somatic embryogenesis and polyembryogenesis was achieved only in media devoid of the antibiotic. Genetic transformation was confirmed by the presence of the structural gene through DNA‐DNA hybridization and the enzymatic assay showed its functional expression. Mesophyll protoplasts, leaf calli and S1 progeny of transformed plants grew in the presence of kanamycin (100 μgml ‐1 ). Results are discussed in relation to the use of kanamycin‐resistant plants in somatic hybridization in the genus Medicago .