Premium
Medium‐Genotype‐Interaction on Androgenetic Haploid Production in Wheat
Author(s) -
Fadel F.,
Wenzel G.
Publication year - 1990
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1990.tb01286.x
Subject(s) - plantlet , biology , stamen , ploidy , cultivar , maltose , botany , genotype , poaceae , horticulture , sucrose , tissue culture , food science , gene , genetics , pollen , in vitro
The present study investigates the effects of media on the rate of green plantlet formation from anther cultures of five different winter and spring wheat cultivars. An increased number of embryos and calli were produced on Ficoll‐containing liquid potato‐2 medium, whereas the addition of 0.2 M/l maltose increased the rate of regeneration. The genotypes had strong effect on the formation of plantlets, but the developed procedure allows also the regeneration of recalcitrant genotypes. On the best medium sequence, a total of 2430 anthers over all genotypes were plated which developed 1325 macroscopic structures (55 %) of which 159 green plants have developed, i.e. 12 % from the structures and 7 % from the plated anthers, respectively.