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In‐vitro Fertilization of Ovules of Some Species of Brassicaceae *
Author(s) -
Zenkteler M.
Publication year - 1990
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1990.tb01199.x
Subject(s) - biology , ovule , brassica oleracea , botany , brassicaceae , human fertilization , embryo rescue , brassica , pollination , pollen , agronomy
The method of in‐vitro fertilization of ovules can be successfully applied to various species of Brassincaceae . Mature embryos and plants were obtained after in‐vitro pollinating the ovules of Arabis caucasica, Brassica napus, B. oleracea var. sabellica (kale), B. oleracea var. italica (broccoli), Diplotaxis tenuifolia, Moricandia arvensis and Sisymbrium Loeselli. In the case of Sinapis alba fertilization and embryo development did not occur. The same method has been successfully used for obtaining hybrid immature embryos at different stages of development from crosses between B. napus X D. tenuifolia, B. napus X M. arvensis, B. oleracea var. italica X D. tenuifolia, D. tenuifolia x B. napus, D. tenuifolia. X M. arvensis and D. tenuifolia X S. Loeselli. The present findings show that in‐vitro pollination of ovules of various species of Brassicaceae makes it possible to (a) perform the whole process of fertilization and embryogenesis; and (b) obtain intergeneric hybrid embryos.