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Isoenzymes and their Importance for Breeding Autopolyploids
Author(s) -
Skiebe K.,
Seliger Petra
Publication year - 1990
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1990.tb00462.x
Subject(s) - biology , isozyme , genetics , allele , loss of heterozygosity , locus (genetics) , ploidy , alcohol dehydrogenase , polyploid , gene , enzyme , biochemistry
Isoenzyme analyses have been carried out in polyploid rye. Taking into consideration zymograms at the diploid level, conclusions can be drawn concerning the genetics of band positions and band intensity. This is rather clear with the aspartate aminotransferase and the NAD‐specific aromatic alcohol dehydrogenase. For both isoenzymes one gene locus with two alleles is responsible in the zones analyzed. In both cases a superdominance is assumed. Thereby nulliplex‐, simplex‐, duplex‐, triplex‐ and quadru‐plex types can be distinguished. For the banding pattern with the leucine aminopeptidase two gene loci with two alleles each are responsible. With allelic interaction codominance is supposed. On this base it is possible to differentiate between homozygosity and heterozygosity. Difficulties in autopolyploid breeding can be overcome by means of genetically‐analyzed isoenzymes as markers. This is demonstrated by examples.