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Screening of Rice Varieties for Endosperm Storage Proteins
Author(s) -
Bhowmik A.,
Omura T.,
Kumamaru T.
Publication year - 1990
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1990.tb00461.x
Subject(s) - endosperm , prolamin , glutelin , storage protein , oryza sativa , biology , polyacrylamide gel electrophoresis , gel electrophoresis , japonica , poaceae , sodium dodecyl sulfate , botany , biochemistry , gene , enzyme
A total of 118 rice varieties/lines ( Oryza sativa L.) from Bangladesh were analyzed for endosperm storage proteins on a single seed basis. The screening was done by observing the profiles of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) with standard japonica variety ‘Kinmaze’. Six groups were classified on the basis of the poly‐peptide banding patterns. Two‐dimensional gel electrophoresis was performed to ensure the SDS‐PAGE results in selected varieties. The analysis of extracted protein fractions revealed that one variety had low prolamin and one variety had high glutelin content.