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Effects of Growth Regulator and Genotype on Production of Wheat and Triticale Polyhaploids from Anther Culture *
Author(s) -
Hassawi Dhia S.,
Qi Jiahua,
Liang G. H.
Publication year - 1990
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1990.tb00400.x
Subject(s) - kinetin , picloram , biology , triticale , dicamba , callus , auxin , cultivar , 1 naphthaleneacetic acid , botany , zeatin , gibberellic acid , doubled haploidy , horticulture , explant culture , cytokinin , ploidy , agronomy , biochemistry , germination , in vitro , weed control , gene
Homozygous doubled‐haploid plantlets derived from anther culture of wheat ( Triticum aestivum L.) and triticale (× Triticosecale Wittmack ) are useful breeding materials. However, efficiency of an‐drogenesis needs improvement. We used media (basic components, are the same as 85DI2) each containing one of the seven auxins [2,4,5‐trichlorophenoxyacetic acid (2,4,5–T), P ‐chloraphenoxyacetic acid (pCPA), 3,6‐dichloro‐o‐anisic acid (dicamba), 4‐amino‐3,5,6‐trichloropicolinic acid (picloram), indole‐3‐butrytic acid (IBA), indole‐3‐acctic acid (IAA), and 2,4‐dichlorophenoxyacetic acid (2,4‐D) as a control] in combination with 6‐furturyl‐aminopurine (kinetin). In addition, each of the four cytokinins [6‐benzylaminopurine (6‐HA), 2‐isopenlylnyl adenine (2‐ip), 6‐(4‐hydroxy‐3‐meihylbut‐2‐enylamino) purinc (zeatin), and kinetin as a control] was tested in combination with 1‐naphthalene acetic acid (NAA). Anthers containing microsporcs at miduninucleatc stage from live wheat cultivars (Angus, Centurk, Chris, K.itt, and Pavon 76) and two octoploid trilicale lines (T81, T82) were tested mainly for callus induction and polyhaploid production on each of the 11 media. The cultivar × medium interaction was not significant, When averaged over all growth regulators, Pavon was (he best cultivar which produced 14.4 % calli and 23 % polyhaploid plantlets. Averaged over all cultivars, the medium containing 2, 4‐U produced the highest calli (13.9 %). Undifferentiated calli were regenerated on 87T1 medium, which contained IAA (1 mg/1) and kinetin (2 mg/1).