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Hybridization of Sinapis alba L. and Brassica napus L. via Embryo Rescue
Author(s) -
Ripley Van L.,
Arnison P. G.
Publication year - 1990
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1990.tb00398.x
Subject(s) - biology , embryo rescue , brassica , backcrossing , sinapis , hybrid , pollen , rapeseed , botany , vernalization , embryo , white mustard , hybrid seed , cultivar , horticulture , interspecific hybridization , gene , photoperiodism , genetics
Embryo rescue techniques were used to obtain hybrids between Sinapis alba L. (white mustard) and Brassica napus L. (oilseed rape) with the goal of improving the disease tolerance of oilseed rape. Hybrid plants with 31 or 43 chromosomes were only recovered, when S. alba , was used as the female parent. One hybrid was obtained from the cross S. alba L. cv. ‘Kirby’× B. napus L. cv. ‘Topas’, while 26 hybrids were obtained, when various S. alba L. cultivars were pollinated with the rapid cycling B. napus line CrGC 5006. All F 1 , hybrid plants were male sterile; however, the first generation backcross to B. napus L., also obtained by embryo rescue, produced plants with 50 chromosomes and 61–84 % pollen viability. Second backcross generation seed was produced by normal sexual crossing. Preliminary cytological analyses of pollen mother cells of hybrid plants suggests the possibility of genetic exchange between the two species.