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Comparisons of Isozyme Patterns in Resynthesized Amphihaploid Rapeseed ( Brassica napus ) and Their Parental Species Brassica campestris and Brassica oleracea
Author(s) -
Mündges Hiltrud,
Diederichsen Elke,
Köhler W.
Publication year - 1989
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1989.tb00382.x
Subject(s) - brassica , biology , rapeseed , brassica oleracea , isozyme , ploidy , botany , cultivar , enzyme , genetics , gene , biochemistry
Resynthesized rapeseed plants produced by embryo culture and the progeny of their selfed parents from Brassica oleracea and Brassica campestris were analyzed by starch gel electrophoresis staining of enzymes GPI, LAP and SDH. In at least one unequivocal case it could be proved for each enzyme system studied that the alleles of both parents arc independently expressed in the newly synthesized individuals and that in the pattern of the dimeric enzyme GPI interlocus bands of the homoeologous loci arc visible. Comparisons with enzyme patterns in cultivated B. napus leads to the conclusion that in general active enzyme loci of both parental genomes are present. This is of great significance in estimating slide frequencies, degree of heterozygosity and other genetic parameters of B. nupus populations/cultivars.