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Sexual Transmission of Somatic Embryogenesis in Dactylis glomerate *
Author(s) -
Gavin Anita L.,
Conger B. V.,
Trigiano R. N.
Publication year - 1989
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1989.tb00380.x
Subject(s) - somatic embryogenesis , biology , dactylis glomerata , botany , genotype , tissue culture , embryo , poaceae , embryogenesis , genetics , in vitro , gene
A Dactylis glomerata L. genotype that produces somatic embryos in vitro was tested for the ability to sexually transmit the embryogenic trait. Reciprocal crosses were performed between the embryogenic and two non‐embryogenic genotypes. Leaf segments from 69 F 1 plants were cultured on Schenk and Hildebrandt medium amended with 30 μM 3,6‐dichloro‐o‐anisic acid (dicamba). Somatic embryogenesis was expressed in 39 of the F 1 plants. The embryogenic parent was female for 18 of these plants and male for the other 21. The 39:30 ratio of embryogenic: non‐embryogenic fits an expected 1:1 for tetrasomic inheritance of a dominant nuclear gene.