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A Simple Method of Inducing Somatic Embryogenesis in Brassica juncea (L.) Czern & Coss.
Author(s) -
Kirti P. B.,
Chopra V. L.
Publication year - 1989
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1989.tb00317.x
Subject(s) - brassica , biology , explant culture , somatic embryogenesis , hypocotyl , botany , shoot , inoculation , murashige and skoog medium , micropropagation , tissue culture , horticulture , in vitro , genetics
A single‐step method for the induction and development of somatic embryoids from hypocotyls explains of Brassica juncea is reported. On modified MS medium containing 2 % sucrose, 0.25 mgl 1 2,4‐D, 0.5 mgl 1 each of NAA and BaP‐R, each explant calluses at both of and at its best, 31% of explants produce embryoids. In the variety RLM‐198, the number of embryoids ranges from 8–21 per culture. Each embryoid, upon proliferation, developed up to the 25 shoots. The method is rapid; the time La ken from inoculation to the development of intact plantlets is 8–10 weeks. Regenerated plants have flowered normally and have set seed. The system can profitably be used for in vitro mutant selection and early bulking in mustard.