Premium
Genetic Analysis of DIA, GOT and PGI Isozyme Loci in Daucus carota L. ssp. sativas
Author(s) -
Westphal L.,
Wricke G.
Publication year - 1989
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1989.tb00314.x
Subject(s) - isozyme , biology , allele , daucus carota , locus (genetics) , genetics , null allele , gene , botany , enzyme , biochemistry
Isozymes are useful biochemical markers in plant breeding. This paper presents the first results on the inheritance of three enzyme systems: diaphorase (DIA), glutamate oxalacetate transaminase (GOT) and phosphoglucoisomerase (PGI) from leaves of Daucus carota L. ssp. sativus and the linkage relationships among these isozyme loci, marked Dia , Got , and Pgi . Methods of extraction and electrophoresis are described. Segregation ratios indicated that the DIA system is controlled by three genes: Dia‐1, Dia‐2 and Dia‐3, Dia‐1 as invariant. Two codominant alleles and a null allele were found for Dia‐2 , and four codominant alleles for Dia‐3 ‐GOT isozymes are encoded by three genes; Got‐1, Got‐2 and Got‐3 . An active allele and a null allele were observed for Got‐1 , two codominant alleles for Got‐2 and Got‐3 , respectively. PGI isozymes are controlled by a single gene, Pgi‐1 , with two codominant alleles. Altogether one invariant isozyme locus and six polymorphic loci are postulated. Test of joint segregation suggested a linkage of Dia‐3 and Pgi‐1 . The recombination value is 0.04 ± 0.009.