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Regeneration of Pea ( Pisum sativum L.) Plantlets by in vitro Culture of Immature Embryos
Author(s) -
Natali L.,
Cavallini A.
Publication year - 1987
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1987.tb01168.x
Subject(s) - plantlet , biology , pisum , sativum , organogenesis , botany , regeneration (biology) , embryo , embryo culture , shoot , tissue culture , murashige and skoog medium , in vitro , embryogenesis , microbiology and biotechnology , genetics , gene
Plant regeneration was achieved from immature embryo‐derived, calli of Pisum sativum. Embryo axes were separated from cotyledons and cultured on different media containing BAP and NAA until plantlet regeneration. Rooting of the plantlets was obtained on MS medium supplemented with 2 mg/1 IBA. Frequency of regeneration was shown to be under the influence of the genotype. Histological preparations showed de novo origin of the shoots via organogenesis. Out of 2C regenerated plantlets, 11 were diploids (2n = 14) arid 9 aneusomatic (chromosomal mosaics) with chromosome numbers ranging from 12 to 16.