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Comparison of Anther Culture and Hordeum bulbosum Method for the Production of Doubled Haploid in Winter Barley I. Production of Green Plants
Author(s) -
Devaux P.
Publication year - 1987
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1987.tb01119.x
Subject(s) - doubled haploidy , stamen , biology , hordeum vulgare , ploidy , hordeum , hybrid , poaceae , botany , agronomy , horticulture , gene , pollen , genetics
The relative response to two‐major techniques of producing haploids in barley – anther culture and the Hordeum bulbosyum method – were compared using seven F 1 hybrids from a winter barley breeding programme. Both techniques were Applied to the same plants with the same number of replications. 358 viable green plants were obtained from 420 spikes ot donor plants used: 147 through anther culture and 211 through the H. bulbosum method. From the data analysis we found that the response to the two techniques was not significantly different, genotype effect and genotype × technique interaction were highly significant. The possibility of combining the two techniques in a doubled haploid production programme is discussed.