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Variation in Nicotine Content of Cultured Cell Lines of Nicotiana Species and their Somatic and Sexual Hybrids
Author(s) -
Sabour M.,
Simmonds J.,
Setterfield G.
Publication year - 1986
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1986.tb01073.x
Subject(s) - biology , hybrid , nicotine , somatic cell , heterosis , nicotiana tabacum , botany , genetics , gene , neuroscience
Several Nicotiana species, their intergeneric sexual and somatic hybrids were compared for their in vitro growth rate and nicotine content. Cell yield on a dry weight basis and HPLC analysis of their nicotine content revealed a very high variation within each cell line from parental, sexual and somatic hybrids for the two traits analyzed. The trigeneríc sexual hybrids (N. rustica × N. debneyi) × N. tabacum and only two of the N. rustica × N. tabacum somatic hybrid lines show a positive heterosis in nicotine content in vitro The in vitro growth rate and nicotine content did not demonstrate a statistically significant correlation.