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Genotyping of Cryptococcus neoformans isolated from captive birds in Uberaba, Minas Gerais, Brazil
Author(s) -
FerreiraPaim Kennio,
AndradeSilva Leonardo,
Mora Delio Jose,
Pedrosa André Luiz,
Rodrigues Virmondes,
SilvaVergara Mario León
Publication year - 2011
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2010.01901.x
Subject(s) - cryptococcus neoformans , biology , cryptococcus , genotyping , restriction fragment length polymorphism , genotype , veterinary medicine , cryptococcus gattii , mating type , amplified fragment length polymorphism , typing , microbiology and biotechnology , genetic diversity , genetics , population , medicine , environmental health , gene
Summary To evaluate Cryptococcus spp. molecular types isolated from captive birds’ droppings, an epidemiological survey was carried out in Uberaba, Minas Gerais, Brazil, from December 2006 to September 2008. A total of 253 samples of bird excreta (120 fresh and 133 dry) were collected from pet shop cages and houses in different neighbourhoods. Cryptococcus neoformans was isolated in 19 (14.28%) dry samples and one fresh sample (0.84%). Cryptococcus laurentii was recovered from seven (5.26%) dry samples, but not in the fresh samples. The canavanine–glycine–bromothymol blue test was positive in all but one of the C. laurentii isolates. Cryptococcus neoformans molecular typing was performed using URA 5‐RFLP and the mating type locus using mating type specific PCR. Nineteen (95.0%) presented genotype VNI and one VNII (5.0%). In addition, all isolates presented mating type α. Thus, the genotype of the environmental C. neoformans isolates observed in this study is in accordance with others already reported around the world and adds information about its distribution in Brazil. Cryptococcus laurentii strains were typed using URA 5‐RFLP and M13 fingerprinting, which showed similar profiles among them. Thus, despite the low number of C. laurentii isolates analysed, their molecular profile is different from another already reported.