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Photodynamic inactivation of Candida albicans by BAM‐SiPc
Author(s) -
So CheungWai,
Tsang Paul W. K.,
Lo PuiChi,
Seneviratne C. J.,
Samaranayake Lakshman P.,
Fong WingPing
Publication year - 2010
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2008.01688.x
Subject(s) - candida albicans , clonogenic assay , photodynamic therapy , incubation , viability assay , intracellular , chemistry , antifungal , reactive oxygen species , oxygen , cell , biophysics , microbiology and biotechnology , biology , biochemistry , organic chemistry
Summary Photodynamic therapy is a treatment that combines the use of three non‐toxic components, viz. photosensitiser, light and oxygen to cause localised oxidative photodamage. In the present study, the antifungal effect of the photosensitiser, BAM‐SiPc, an unsymmetrical bisamino phthalocyanine, was investigated. BAM‐SiPc was effective in photo‐inactivating Candida albicans in a dose‐dependent manner. The cell viability as determined by the clonogenic assay was reduced to c. 10% at 0.02 μmol l −1 BAM‐SiPc with a total fluence of 12 J cm −2 at a cell density of 10 7 cells ml −1 . A short incubation time of 5–15 min was sufficient to allow the photosensitiser to exert its optimal antifungal activity. Microscopical analysis showed that BAM‐SiPc was effectively internalised by the fungal cells. Photodynamic treatment led to an increase in the intracellular reactive oxygen species level and disturbed the membrane integrity of the fungal cells.