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Is the geographical origin of a Paracoccidioides brasiliensis isolate important for antigen production for regional diagnosis of paracoccidioidomycosis?
Author(s) -
Batista Jr João,
De Camargo Zoilo Pires,
Fernandes Geisa Ferreira,
Vicentini Adriana Pardini,
Fontes Cor Jesus Fernandes,
Hahn Rosane Christine
Publication year - 2010
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2008.01687.x
Subject(s) - paracoccidioidomycosis , paracoccidioides brasiliensis , immunodiffusion , paracoccidioides , biology , antigen , serology , microbiology and biotechnology , ouchterlony double immunodiffusion , immunology , antibody , antiserum
Summary In this study, exoantigens produced from two Paracoccidioides brasiliensis strains isolated in two different geographical areas were compared in terms of sensitivity and specificity in relation to paracoccidioidomycosis (PCM) diagnosis. Exoantigens from P. brasiliensis 550B (Ag 550B) isolated in the central‐west region of Brazil (Mato Grosso State) and exoantigen produced from P. brasiliensis B‐339 (Ag B‐339) used in reference laboratories were compared by immunodiffusion (ID) tests. When Ag 550B was used in ID test against sera of patients from Mato Grosso and São Paulo, positivity was 92.3% and 41.3%, respectively. On the other hand, when Ag B‐339 was tested with the same sera, positivity was 26.2% and 100%, respectively. These results suggest that differences in the antigenic composition probably related to phylogenetic peculiarities in P. brasiliensis isolates from the central‐western region of Brazil should be considered in the diagnosis of PCM.