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Optimization of bioassay method for the quantitative microbiological determination of amphotericin B
Author(s) -
Fittler András,
Kocsis Béla,
Gerlinger Imre,
Botz Lajos
Publication year - 2010
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2008.01660.x
Subject(s) - bioassay , candida albicans , amphotericin b , chromatography , agar , microbiology and biotechnology , methylene blue , chemistry , corpus albicans , antifungal , biology , bacteria , biochemistry , genetics , photocatalysis , catalysis
Summary The aim of this study was to find the optimal bioassay parameters for the quantitative analysis of an amphotericin B nasal spray solution as the bioassay conditions recommended by the Ph. Eur. 6. were less sensitive and were only applicable for the measurement of a narrow concentration range, which makes the method unsuitable in case of a stability test. We evaluated five commonly used assay media with Candida albicans and Saccharomyces cerevisiae as test organisms. Our results showed that Mueller Hinton Agar supplemented with 2% glucose and 0.5 μg ml −1 methylene blue inoculated with C. albicans gave the best bioassay circumstance as a wide concentration range (1.54–60.0 μg ml −1 amphotericin B) could be measured and the inhibition zone borders were distinct and easy to read.