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Development of TaqMan real‐time polymerase chain reaction for the detection and identification of Penicillium marneffei
Author(s) -
Pornprasert Sakorn,
Praparattanapan Jutarat,
Khamwan Chantana,
Pawichai Sudjai,
Pimsarn Parichat,
Samleerat Tanawan,
Leechanachai Pranee,
Supparatpinyo Khunchai
Publication year - 2009
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2008.01653.x
Subject(s) - penicillium marneffei , taqman , dimorphic fungus , polymerase chain reaction , biology , real time polymerase chain reaction , microbiology and biotechnology , yeast , fungus , virology , gene , genetics , human immunodeficiency virus (hiv) , coinfection , botany
Summary Penicillium marneffei is a dimorphic fungus, which is endemic in Southeast Asia and responsible for emerging opportunistic infections. Diagnosis of penicilliosis may be difficult when few yeast cells are present, while a gold standard diagnosis technique requires long‐term culture. In order to provide a more rapid and accurate diagnosis, we developed a TaqMan real‐time PCR to detect and identify P. marneffei DNA coding for 5.8S rRNA in purified yeast DNA and clinical samples. All P. marneffei DNA preparations could be detected using specific primers and TaqMan probe. The assay has a sensitivity to detect at least 10 yeast cells in seeded blood. Moreover, it can detect P. marneffei DNA in peripheral blood samples and blood‐culture bottles. Therefore, the real‐time PCR assay may represent a potential tool for early diagnosis of penicilliosis marneffei.