Premium
Genetic variability of Candida albicans isolates in a university hospital in Hungary
Author(s) -
Farkas Zoltán,
Kocsubé Sándor,
Tóth Mónika,
Vágvölgyi Csaba,
Kucsera Judit,
Varga János,
Pfeiffer Ilona
Publication year - 2009
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2008.01627.x
Subject(s) - rapd , candida albicans , biology , corpus albicans , mitochondrial dna , restriction fragment length polymorphism , genetics , genetic variability , population , microbiology and biotechnology , genetic variation , genotype , genetic diversity , gene , medicine , environmental health
Summary The occurrence and genetic variability of Candida albicans isolates in a Hungarian hospital were examined. Among the 103 Candida isolates, 44 (42.7%) proved to be C. albicans species. Comparing with a previous study carried out in 2002, the percentage of infections caused by C. albicans decreased in Hungary in this period with an increasing incidence of non‐albicans species, in accordance with the world‐wide trend. The genetic variability of the isolates was examined using mitochondrial DNA (mtDNA), restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) analysis and electrophoretic karyotyping. The examined C. albicans isolates could be clustered into four groups based on their mtDNA profiles. The electrophoretic karyotypes of the isolates were mostly identical to that of the reference strain 1006, with the exception of mtDNA type II isolates. RAPD analy s is could be used to cluster the isolates into different groups, but this clustering was not in complete agreement with their assignment to mtDNA types. Population genetic analyses of the data indicated low amounts of recombination among these C. albicans strains. None of the isolates exhibited decreased susceptibilities to 5‐fluorocytosine.