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Genotyping of Sporothrix schenckii by analysis of ribosomal DNA regions
Author(s) -
Zhang Zhenying,
Liu Xiaoming,
Yang Guoling,
Gao Xiaorong,
Jin Liji,
An Lijia
Publication year - 2006
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2006.01256.x
Subject(s) - sporothrix schenckii , biology , southern blot , genomic dna , genotype , ribosomal dna , sporotrichosis , internal transcribed spacer , genotyping , typing , restriction enzyme , genetics , dna , restriction fragment length polymorphism , sporothrix , polymerase chain reaction , amplified ribosomal dna restriction analysis , restriction map , ribosomal rna , microbiology and biotechnology , phylogenetic tree , gene , plasmid , immunology
Summary An attempt was made to identify the genotypes of 31 Sporothrix schenckii strains and the relationship between the genotypes, the geographic distributions and clinical manifestations using restriction fragment length polymorphic analysis. The total DNA was extracted by cetyltrimethyl ammonium bromide. The polymorphisms were detected by hybridisation of Apa I‐digested Sporothrix genomic DNA with a probe amplified from the small subunit ribosomal DNA and adjacent internal transcribed spacer regions. The band patterns manifested by Southern blotting were explored to investigate the genotypes of the 31 strains of S. schenckii collected from five different areas in China. Of the data from the 31 strains of S. schenckii studied, 15 individual patterns (DNA type A–O) were recognised. Type A–C accounted for 51.61% of all the strains. The DNA typing of S. schenckii by Southern blotting was highly sensitive and highly distinguishable. We also found a significant correlation between DNA patterns and different geographic areas and clinical manifestations.