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Use of magnetic beads to extract fungal DNA
Author(s) -
Faggi E.,
Pini G.,
Campisi E.
Publication year - 2005
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2004.01030.x
Subject(s) - microsporum canis , cryptococcus neoformans , dna extraction , genomic dna , microsporum gypseum , polymerase chain reaction , epidermophyton floccosum , trichophyton rubrum , microbiology and biotechnology , biology , trichophyton , dna , candida albicans , genetics , gene , antifungal
Summary Authors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans , belonging to the yeast group and Microsporum canis , M. gypseum , Trichophyton rubrum , T. interdigitale , T. ajelloi , Epidermophyton floccosum , belonging to the dermatophytes group. Extraction products underwent polymerase chain reaction (PCR) fingerprinting with the appropriate primers to point out any disagreement in the genomic profiles. After having determined that the genomic profiles obtained from the DNA extracted from the same strain with the two methods correspond perfectly, the authors concluded that the extraction method with magnetic beads from fungal cells is simpler and quicker than with P/C extraction, greatly facilitating the obtainment of fungal DNA.