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Prevalence of Candida dubliniensis among the stored vaginal Candida isolates in a Turkish hospital
Author(s) -
Acikgoz Z. C.,
Sancak B.,
Gamberzade S.,
Misirlioglu M.
Publication year - 2004
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2004.01012.x
Subject(s) - candida dubliniensis , agar , microbiology and biotechnology , chlamydospore , biology , candida albicans , germ tube , fungemia , internal transcribed spacer , strain (injury) , corpus albicans , ribosomal rna , bacteria , spore , antifungal , genetics , gene , anatomy
Summary In this study, 600 stored Candida species, isolated from vaginal samples of immunocompetent women, and phenotypically identified as C. albicans on the basis of a positive germ tube test, were screened for the presence of C. dubliniensis by three phenotypical methods. Only one strain (0.17%) failed to grow at 45 °C, and produced abundant chlamydospores on both the cornmeal‐Tween 80 agar and the Staib agar. This strain was identified as C. dubliniensis by using the ID‐32C kit (bioMerieux Vitek) and confirmed by DNA sequencing of internal transcript spacer (ITS) region.