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Influence of voriconazole and fluconazole on reconstituted multilayered oesophageal epithelium infected by Candida albicans
Author(s) -
Bernhardt J.,
Bernhardt H.,
Knoke M.,
Ludwig K.
Publication year - 2004
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2004.01004.x
Subject(s) - candida albicans , fluconazole , voriconazole , hypha , microbiology and biotechnology , epithelium , penetration (warfare) , corpus albicans , biology , in vivo , mycosis , nystatin , pathology , antifungal , medicine , immunology , operations research , engineering
Summary Reconstituted multilayered oesophageal epithelium appears to be a good basis to test the efficacy of voriconazole (VOR) and fluconazole (FLU) in the tissue. The resulting model of a Candida oesophagitis was approaching the in vivo situation. We infected the tissue with 2 × 10 6 cfu of the Candida albicans strain SC5314. In the trials with FLU we also used clinical strains. Four hours after infection a good growth of C. albicans appeared mainly with hyphae on the surface of the tissue and a tendency to invasion. The destruction of the tissue began after 36 h. VOR (2 and 16 μ g ml −1 , respectively) prevented the penetration of hyphae into the tissue, when it was given 4–8 h after infection. It was less effective in reduction of Candida growth on the tissue surface. When VOR was given 16–24 h postinfection, the Candida infiltration stopped more slowly. Thirty‐six hours after infection VOR application could not stop the destruction of the tissue despite reducing the fungi. The results with FLU (32 μ g ml −1 ) were in principle the same, but not so distinct. FLU seems to be more effective against clinical strains of C. albicans than against the type strain.