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Agar sublimation test for the in vitro determination of the antifungal activity of morpholine derivatives
Author(s) -
Polak A.,
Jäckel A.,
Noack A.,
Kappe R.
Publication year - 2004
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2004.00975.x
Subject(s) - econazole , caspofungin , terbinafine , clotrimazole , chemistry , microbiology and biotechnology , morpholine , miconazole , resazurin , bifonazole , agar , nystatin , candida albicans , agar diffusion test , pharmacology , amphotericin b , antifungal , biology , itraconazole , biochemistry , organic chemistry , genetics , escherichia coli , bacteria , gene
Summary We studied the in vitro antifungal activities of a wide range of antimycotic agents, including amorolfine, terbinafine, naftifine, five morpholine derivatives, ciclopiroxolamine, bifonazole, clotrimazole, ketoconazole, itraconazole, fluconazole, voriconazole, flucytosine, amphotericin B, nystatin, and caspofungin, against Candida albicans and Trichophyton rubrum by conventional agar diffusion tests and by a novel sublimation method. For the sublimation method, 6 mm filter paper disks were soaked with defined amounts of antimycotic drugs, air dried, placed in the center of the lids of 9 cm Petri dishes, and incubated upside down with inoculated agar plates 10 mm above the disks. The conventional disk diffusion tests produced inhibition zones as previously described. The disk sublimation tests produced large inhibition zones with amorolfine, five amorolfine derivatives, and terbinafine, but with none of the other antifungal agents. Possible therapeutic advantages of agents, which are able to overcome air cavities in mycotic lesions, e.g. in onychomycosis, are discussed.