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Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sources
Author(s) -
Abuodeh R. O.,
Chester E. M.,
Scalarone G. M.
Publication year - 2004
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2004.00972.x
Subject(s) - blastomyces dermatitidis , blastomycosis , antigen , blastomyces , serology , histoplasmosis , histoplasma , lysis , microbiology and biotechnology , biology , cross reactivity , yeast , virology , immunology , histoplasma capsulatum , antibody , cross reactions , biochemistry
Summary Yeast phase lysate antigens from 10 isolates of Blastomyces dermatitidis (dog, ERC‐2 and T‐58; T‐27, polar bear; woodpile, ER‐3; bat lung, 48938; human, B5929, B5895, B5896, B5931, and CAPP) from different geographical regions, in addition to a Histoplasma capsulatum (G217B) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme‐linked immunosorbent assay. In addition, the lysate antigens were also assayed against histoplasmosis‐positive human serum samples to study their cross‐reactivity. Variable results were obtained with T‐58 and T‐27 exhibiting the greatest reactivity. We also noticed that the lysate did not react consistently to serum samples across species with lesser reactivity evidenced when testing dog sera. Finally, T‐58 gave the highest cross‐reactivity with histoplasmosis‐positive sera. The study may prove valuable in the development of antigen candidates for blastomycosis serodiagnosis.