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PHENOTYPIC AND MOLECULAR DIVERSITY AMONG ENVIRONMENTAL AND CLINICAL ISOLATES OF ASPERGILLUS FUMIGATUS GROUP
Author(s) -
Florio S.,
Picone C.,
Damiani G.,
Guglielminetti M.
Publication year - 2002
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2002.tb04599.x
Subject(s) - humanities , aspergillus fumigatus , biology , art , botany
Our aim was to investigate the morphological and molecular variability of both environmental and clinical isolates of thehpergillus fumigutw group. In fact, this group includes fungi as inhabitants of soil and also etiologic agents of different diseases. Fourteen strains (7 from environment, and 7 from patients) were analysed by means of optical microscope on slide cultures, PCR for the amplification of the alkaline protease gene and RAPD fingerprinting with four random primers [I]. Morphological studies revealed that both environmental and clinical strains belong to the three species of the group: A.fumigutus Fresenius, A. duricuulis, and A. uiridi-nutuns. Molecular characterisation did not reveal any significant polymorphism between clinical and environmental isolates and among the three different species. The obtained results suggest that all strains are potentially pathogenic, according to the finding of other authors [2]. Moreover the observed interspecific morphological variability of the Aspergillus fumigutw group does not correspond to variability at the molecular level.